BIRLA INSTITUTE OF SCIENTIFIC RESEARCH
BISR-PRIMER
Enter or Paste the Sequence in plain or fasta format:
Or Upload the Sequence file (plain or gb or fasta)
:
Pick Reverse Primer:
Pick Forward primer:
Sequence ID
Targets
From:
How Many:
(Enter how many nucleotide from target position)
Excluded Region
From:
How Many:
(Enter how many nucleotide exclude from target position)
Product Size Range
From
100
300
500
700
900
1100
1300
To:
300
500
700
900
1100
1300
Number of Return:
General Primer picking conditions:
Chose Primer Length:
Min:
Opt:
Max:
Chose Primer Tm :
Min:
Opt:
Max:
Product Tm :
Min:
Opt:
Max:
Chose Primer GC% :
Min:
Opt:
Max:
Maximum self complementarity:
Maximum 3' self complementarity:
Maximum unknown bases:
Maximum poly X :
First base index:
GC clamp:
Salt Concentration:
Oligo Concetration:
Included region : From
upto:
(Enter how many nucleotide from target position)
Start codon position:
